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pcdna3 1 ebov gp ha  (Addgene inc)


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    Addgene inc pcdna3 1 ebov gp ha
    Pcdna3 1 Ebov Gp Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+ebov+gp+ha/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/pm36224200-430-105-133
    Average 96 stars, based on 2723 article reviews
    pcdna3 1 ebov gp ha - by Bioz Stars, 2026-09
    96/100 stars

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    Expressing:

    Article Title: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy.
    Article Snippet: Expression vectors pcDNA3.1-EBOV-GP, pcDNA3.1-EBOV-GPΔMLD, pCMV3-EBOV-VP40, pNL-Luc-△Env, pMSCVneo-CANX-HA, pCMV6-CALR-Myc, and pCAGGS-CANX-HA were reported25. pcDNA3.1-GFP-FLAG-HA, pRK5Ub-His, pCAGGS-PDIA3-Myc, pCAGGS-PDIA3-HA, pcDNA3.1 vectors expressing EBOV-GP, GP△MLD, sGP or ssGP with a N-terminal HiBiT tag, pCAGGS-MARV-GP with a C-terminal HiBiT tag, pcDNA3.1 vectors expressing GPs from BDBV, RETV, SBOV, or TAFV with a HA-FLAG-tag, and vectors expressing HIV-1 Env, IAV-H5 HA, and VSV-G were reported24. pCAGGS-MARCH8-HA and its W114A mutant were reported26. pEGFPC1 was from Takara. pCMV6-CANX-Myc-FLAG was constructed from pCAGGS-CANXHA by PCR and ASiSI/MluI digestion. pCAGGS vectors expressing MERS-CoV-S, SRAS-CoV-S, and SARS-CoV2-S with a FLAG-tag were constructed by PCR and EcoRI/XhoI digestion. pEGFPN1 vectors expressingCALR, TGLON2, andEBOV-GPwereconstructedby PCRand XhoI/BspEI digestion. pcDNA3.1-EBOV-GP-HA was constructed by PCR and NheI/HindIII digestion. pmBFPN1-CALR was constructed from pEGFPN1-CALR by replacing EGFP with mBFP via AgeI/NotI digestion. pRK5-HA-Ub-WT was obtained from Ted Dawson via Addgene (#17608). pCMV6-RNF26-Myc-FLAG, pCMV6-RNF185-Myc-FLAG, and pcDNA3.1-TRIM25-FLAG were ordered from CoME, ORIGENE, or JINSIRUI. pCAGGS-RNF26-mCherry was constructed from pCAGGS-ACE2mCherry by PCR and EcoRI digestion followed by homologous recombination (Vazyme, C113-01). pcDNA3.1-RNF26-VN-HA was constructed from pcDNA3.1-ACE2-VN-HA by PCR and XhoI/BspEI digestion. pcDNA3.1-FLAG-EBOV-GP-FLAG-VC was constructed from pcDNA3.1-ACE2-FLAG-VCbyPCR andXhoI/BspEI digestion.

    Mutagenesis:

    Article Title: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy.
    Article Snippet: Expression vectors pcDNA3.1-EBOV-GP, pcDNA3.1-EBOV-GPΔMLD, pCMV3-EBOV-VP40, pNL-Luc-△Env, pMSCVneo-CANX-HA, pCMV6-CALR-Myc, and pCAGGS-CANX-HA were reported25. pcDNA3.1-GFP-FLAG-HA, pRK5Ub-His, pCAGGS-PDIA3-Myc, pCAGGS-PDIA3-HA, pcDNA3.1 vectors expressing EBOV-GP, GP△MLD, sGP or ssGP with a N-terminal HiBiT tag, pCAGGS-MARV-GP with a C-terminal HiBiT tag, pcDNA3.1 vectors expressing GPs from BDBV, RETV, SBOV, or TAFV with a HA-FLAG-tag, and vectors expressing HIV-1 Env, IAV-H5 HA, and VSV-G were reported24. pCAGGS-MARCH8-HA and its W114A mutant were reported26. pEGFPC1 was from Takara. pCMV6-CANX-Myc-FLAG was constructed from pCAGGS-CANXHA by PCR and ASiSI/MluI digestion. pCAGGS vectors expressing MERS-CoV-S, SRAS-CoV-S, and SARS-CoV2-S with a FLAG-tag were constructed by PCR and EcoRI/XhoI digestion. pEGFPN1 vectors expressingCALR, TGLON2, andEBOV-GPwereconstructedby PCRand XhoI/BspEI digestion. pcDNA3.1-EBOV-GP-HA was constructed by PCR and NheI/HindIII digestion. pmBFPN1-CALR was constructed from pEGFPN1-CALR by replacing EGFP with mBFP via AgeI/NotI digestion. pRK5-HA-Ub-WT was obtained from Ted Dawson via Addgene (#17608). pCMV6-RNF26-Myc-FLAG, pCMV6-RNF185-Myc-FLAG, and pcDNA3.1-TRIM25-FLAG were ordered from CoME, ORIGENE, or JINSIRUI. pCAGGS-RNF26-mCherry was constructed from pCAGGS-ACE2mCherry by PCR and EcoRI digestion followed by homologous recombination (Vazyme, C113-01). pcDNA3.1-RNF26-VN-HA was constructed from pcDNA3.1-ACE2-VN-HA by PCR and XhoI/BspEI digestion. pcDNA3.1-FLAG-EBOV-GP-FLAG-VC was constructed from pcDNA3.1-ACE2-FLAG-VCbyPCR andXhoI/BspEI digestion.

    Construct:

    Article Title: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy.
    Article Snippet: Expression vectors pcDNA3.1-EBOV-GP, pcDNA3.1-EBOV-GPΔMLD, pCMV3-EBOV-VP40, pNL-Luc-△Env, pMSCVneo-CANX-HA, pCMV6-CALR-Myc, and pCAGGS-CANX-HA were reported25. pcDNA3.1-GFP-FLAG-HA, pRK5Ub-His, pCAGGS-PDIA3-Myc, pCAGGS-PDIA3-HA, pcDNA3.1 vectors expressing EBOV-GP, GP△MLD, sGP or ssGP with a N-terminal HiBiT tag, pCAGGS-MARV-GP with a C-terminal HiBiT tag, pcDNA3.1 vectors expressing GPs from BDBV, RETV, SBOV, or TAFV with a HA-FLAG-tag, and vectors expressing HIV-1 Env, IAV-H5 HA, and VSV-G were reported24. pCAGGS-MARCH8-HA and its W114A mutant were reported26. pEGFPC1 was from Takara. pCMV6-CANX-Myc-FLAG was constructed from pCAGGS-CANXHA by PCR and ASiSI/MluI digestion. pCAGGS vectors expressing MERS-CoV-S, SRAS-CoV-S, and SARS-CoV2-S with a FLAG-tag were constructed by PCR and EcoRI/XhoI digestion. pEGFPN1 vectors expressingCALR, TGLON2, andEBOV-GPwereconstructedby PCRand XhoI/BspEI digestion. pcDNA3.1-EBOV-GP-HA was constructed by PCR and NheI/HindIII digestion. pmBFPN1-CALR was constructed from pEGFPN1-CALR by replacing EGFP with mBFP via AgeI/NotI digestion. pRK5-HA-Ub-WT was obtained from Ted Dawson via Addgene (#17608). pCMV6-RNF26-Myc-FLAG, pCMV6-RNF185-Myc-FLAG, and pcDNA3.1-TRIM25-FLAG were ordered from CoME, ORIGENE, or JINSIRUI. pCAGGS-RNF26-mCherry was constructed from pCAGGS-ACE2mCherry by PCR and EcoRI digestion followed by homologous recombination (Vazyme, C113-01). pcDNA3.1-RNF26-VN-HA was constructed from pcDNA3.1-ACE2-VN-HA by PCR and XhoI/BspEI digestion. pcDNA3.1-FLAG-EBOV-GP-FLAG-VC was constructed from pcDNA3.1-ACE2-FLAG-VCbyPCR andXhoI/BspEI digestion.

    Polymerase Chain Reaction:

    Article Title: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy.
    Article Snippet: Expression vectors pcDNA3.1-EBOV-GP, pcDNA3.1-EBOV-GPΔMLD, pCMV3-EBOV-VP40, pNL-Luc-△Env, pMSCVneo-CANX-HA, pCMV6-CALR-Myc, and pCAGGS-CANX-HA were reported25. pcDNA3.1-GFP-FLAG-HA, pRK5Ub-His, pCAGGS-PDIA3-Myc, pCAGGS-PDIA3-HA, pcDNA3.1 vectors expressing EBOV-GP, GP△MLD, sGP or ssGP with a N-terminal HiBiT tag, pCAGGS-MARV-GP with a C-terminal HiBiT tag, pcDNA3.1 vectors expressing GPs from BDBV, RETV, SBOV, or TAFV with a HA-FLAG-tag, and vectors expressing HIV-1 Env, IAV-H5 HA, and VSV-G were reported24. pCAGGS-MARCH8-HA and its W114A mutant were reported26. pEGFPC1 was from Takara. pCMV6-CANX-Myc-FLAG was constructed from pCAGGS-CANXHA by PCR and ASiSI/MluI digestion. pCAGGS vectors expressing MERS-CoV-S, SRAS-CoV-S, and SARS-CoV2-S with a FLAG-tag were constructed by PCR and EcoRI/XhoI digestion. pEGFPN1 vectors expressingCALR, TGLON2, andEBOV-GPwereconstructedby PCRand XhoI/BspEI digestion. pcDNA3.1-EBOV-GP-HA was constructed by PCR and NheI/HindIII digestion. pmBFPN1-CALR was constructed from pEGFPN1-CALR by replacing EGFP with mBFP via AgeI/NotI digestion. pRK5-HA-Ub-WT was obtained from Ted Dawson via Addgene (#17608). pCMV6-RNF26-Myc-FLAG, pCMV6-RNF185-Myc-FLAG, and pcDNA3.1-TRIM25-FLAG were ordered from CoME, ORIGENE, or JINSIRUI. pCAGGS-RNF26-mCherry was constructed from pCAGGS-ACE2mCherry by PCR and EcoRI digestion followed by homologous recombination (Vazyme, C113-01). pcDNA3.1-RNF26-VN-HA was constructed from pcDNA3.1-ACE2-VN-HA by PCR and XhoI/BspEI digestion. pcDNA3.1-FLAG-EBOV-GP-FLAG-VC was constructed from pcDNA3.1-ACE2-FLAG-VCbyPCR andXhoI/BspEI digestion.

    FLAG-tag:

    Article Title: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy.
    Article Snippet: Expression vectors pcDNA3.1-EBOV-GP, pcDNA3.1-EBOV-GPΔMLD, pCMV3-EBOV-VP40, pNL-Luc-△Env, pMSCVneo-CANX-HA, pCMV6-CALR-Myc, and pCAGGS-CANX-HA were reported25. pcDNA3.1-GFP-FLAG-HA, pRK5Ub-His, pCAGGS-PDIA3-Myc, pCAGGS-PDIA3-HA, pcDNA3.1 vectors expressing EBOV-GP, GP△MLD, sGP or ssGP with a N-terminal HiBiT tag, pCAGGS-MARV-GP with a C-terminal HiBiT tag, pcDNA3.1 vectors expressing GPs from BDBV, RETV, SBOV, or TAFV with a HA-FLAG-tag, and vectors expressing HIV-1 Env, IAV-H5 HA, and VSV-G were reported24. pCAGGS-MARCH8-HA and its W114A mutant were reported26. pEGFPC1 was from Takara. pCMV6-CANX-Myc-FLAG was constructed from pCAGGS-CANXHA by PCR and ASiSI/MluI digestion. pCAGGS vectors expressing MERS-CoV-S, SRAS-CoV-S, and SARS-CoV2-S with a FLAG-tag were constructed by PCR and EcoRI/XhoI digestion. pEGFPN1 vectors expressingCALR, TGLON2, andEBOV-GPwereconstructedby PCRand XhoI/BspEI digestion. pcDNA3.1-EBOV-GP-HA was constructed by PCR and NheI/HindIII digestion. pmBFPN1-CALR was constructed from pEGFPN1-CALR by replacing EGFP with mBFP via AgeI/NotI digestion. pRK5-HA-Ub-WT was obtained from Ted Dawson via Addgene (#17608). pCMV6-RNF26-Myc-FLAG, pCMV6-RNF185-Myc-FLAG, and pcDNA3.1-TRIM25-FLAG were ordered from CoME, ORIGENE, or JINSIRUI. pCAGGS-RNF26-mCherry was constructed from pCAGGS-ACE2mCherry by PCR and EcoRI digestion followed by homologous recombination (Vazyme, C113-01). pcDNA3.1-RNF26-VN-HA was constructed from pcDNA3.1-ACE2-VN-HA by PCR and XhoI/BspEI digestion. pcDNA3.1-FLAG-EBOV-GP-FLAG-VC was constructed from pcDNA3.1-ACE2-FLAG-VCbyPCR andXhoI/BspEI digestion.

    Homologous Recombination:

    Article Title: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy.
    Article Snippet: Expression vectors pcDNA3.1-EBOV-GP, pcDNA3.1-EBOV-GPΔMLD, pCMV3-EBOV-VP40, pNL-Luc-△Env, pMSCVneo-CANX-HA, pCMV6-CALR-Myc, and pCAGGS-CANX-HA were reported25. pcDNA3.1-GFP-FLAG-HA, pRK5Ub-His, pCAGGS-PDIA3-Myc, pCAGGS-PDIA3-HA, pcDNA3.1 vectors expressing EBOV-GP, GP△MLD, sGP or ssGP with a N-terminal HiBiT tag, pCAGGS-MARV-GP with a C-terminal HiBiT tag, pcDNA3.1 vectors expressing GPs from BDBV, RETV, SBOV, or TAFV with a HA-FLAG-tag, and vectors expressing HIV-1 Env, IAV-H5 HA, and VSV-G were reported24. pCAGGS-MARCH8-HA and its W114A mutant were reported26. pEGFPC1 was from Takara. pCMV6-CANX-Myc-FLAG was constructed from pCAGGS-CANXHA by PCR and ASiSI/MluI digestion. pCAGGS vectors expressing MERS-CoV-S, SRAS-CoV-S, and SARS-CoV2-S with a FLAG-tag were constructed by PCR and EcoRI/XhoI digestion. pEGFPN1 vectors expressingCALR, TGLON2, andEBOV-GPwereconstructedby PCRand XhoI/BspEI digestion. pcDNA3.1-EBOV-GP-HA was constructed by PCR and NheI/HindIII digestion. pmBFPN1-CALR was constructed from pEGFPN1-CALR by replacing EGFP with mBFP via AgeI/NotI digestion. pRK5-HA-Ub-WT was obtained from Ted Dawson via Addgene (#17608). pCMV6-RNF26-Myc-FLAG, pCMV6-RNF185-Myc-FLAG, and pcDNA3.1-TRIM25-FLAG were ordered from CoME, ORIGENE, or JINSIRUI. pCAGGS-RNF26-mCherry was constructed from pCAGGS-ACE2mCherry by PCR and EcoRI digestion followed by homologous recombination (Vazyme, C113-01). pcDNA3.1-RNF26-VN-HA was constructed from pcDNA3.1-ACE2-VN-HA by PCR and XhoI/BspEI digestion. pcDNA3.1-FLAG-EBOV-GP-FLAG-VC was constructed from pcDNA3.1-ACE2-FLAG-VCbyPCR andXhoI/BspEI digestion.



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    pcdna3 1 ebov gp ha  (Addgene inc)
    96
    Addgene inc pcdna3 1 ebov gp ha
    Pcdna3 1 Ebov Gp Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+ebov+gp+ha/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/pm36224200-430-105-133
    Average 96 stars, based on 1 article reviews
    pcdna3 1 ebov gp ha - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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